Improved serodiagnosis of Campylobacter jejuni infections using recombinant antigens
نویسندگان
چکیده
منابع مشابه
Analysis of Campylobacter jejuni antigens with monoclonal antibodies.
To develop monoclonal reagents for antigenic analysis and serotyping of Campylobacter spp., hybridoma cell lines were produced by fusion of mouse myeloma cells and spleen cells from mice immunized with Formalin-treated Campylobacter jejuni organisms. An enzyme immunoassay was used for preliminary screening of the cell culture supernatants and ascites. Twenty-nine clones which reacted with the i...
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Serological tests with crude or recombinant Leishmania antigens are important tools for the diagnosis of leishmania infection. However, these tests are not markers of active visceral leishmaniasis (VL), since antibodies to these markers are often observed in individuals with subclinical L. chagasi infection and they do not fall shortly after therapy. In this study, levels of immunoglobulin G (I...
متن کاملImmunogens of interest for the diagnosis of Campylobacter jejuni infections.
In order to identify the C. jejuni immunogens of interest for the diagnosis of Campylobacter infections, we analyzed the humoral response of 153 patients by using complement fixation (CF) and western blot assays. A first group of 79 sera was from C. jejuni infected patients suffering from enteritis (n=16), Guillain-Barré syndrome (GBS) (n=40) and arthritis (n=23). A second group of 49 sera was ...
متن کاملEvaluation of commercial antisera for serotyping heat-labile antigens of Campylobacter jejuni and Campylobacter coli.
Commercial antisera for serotyping 22 heat-labile antigens of Campylobacter jejuni and Campylobacter coli were evaluated by using 66 isolates from human and nonhuman sources. Test results were compared with results of tests using antisera produced at the Centers for Disease Control (CDC), Atlanta, Ga. All strains (three isolates of each of the 22 serotypes) were typeable with the CDC antisera. ...
متن کاملThe Role of Campylobacter Jejuni in Campylobacteriosis
Campylobacter jejuni is a Gram negative, curved, motile, thermophilic and microaerophilic bacillus in Campylobacteriaceae family, which is one of the main causes of enteritis in human named campylobacteriosis. The main reservoir of Campylobacter jejuni is the alimentary tract of animals, especially chicken and turkey. Consumption of semi-cooked chicken meat, raw milk and unchlorinated water are...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2005
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.46040-0